INTIMATION FOR BODHIKA SEMINAR 15/11/2023
DEPARTMENT OF RASASASTRA AND BHAISHAJYAKALPANA
NAME OF FIRST PRESENTEE:Dr. DIVYA M V
DISSERTATION TITLE: REGENERATIVE POTENTIAL OF SWADAMSTRADI RASAYANA, AMALAKYADI RASAYANA AND THEIR INGREDIENTS IN ZEBRAFISH (Danio rerio)
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Regeneration is a remarkable intrinsic capacity of the human body to restore damaged or lost tissues and functions, thereby maintaining homeostasis and overall health. At the level of the organism, the body has a propensity to go through and acquire changes throughout time, which are typically degenerative. Degeneration, often associated with the gradual deterioration of tissues and organs, is a natural consequence of ageing, and a range of genetic, environmental, and lifestyle factors can further exacerbate it. The intricate balance between degeneration and regeneration processes within the human body in biomedical sciences represents a multifaceted and critical study area. Regenerative medicine focuses on the functional restoration of specific tissues or organs in patients with catastrophic injuries or chronic disease states when their bodies natural regeneration processes fall short of what is required. Enriched with tailored approaches to health and disease, in addition to knowledge of distinctive practices using medicinal plants and peculiar dietary regiments, healthcare systems such as Ayurveda can provide a secure alternative to chronic and degenerative diseases and are increasingly used by individuals with such circumstances. Research opportunities for the biology of regeneration are made possible by the trophorestoration property of the medications and therapies indicated under Ayurvedic Rasayanatantra. Since, zebrafish fins make an excellent study tool for cellular and molecular mechanisms of regeneration, this study aimed to evaluate the regenerative potential of Swadamstradi Rasayana, Amalakyadi Rasayana and their ingredients in the amputated caudal fin of zebrafish. The experimental design was constituted by forty-nine fishes distributed into seven aquaria, receiving six treatments and the seventh one as the control group. Fishes from each treatment were anesthetized and had a portion of their caudal fin amputated with the intention to study the caudal fin's regeneration rates and period. Each experimental setup involved measuring each fish separately before and after amputation, followed by allowing the fish to grow while receiving treatment. To determine the regenerated portions, fishes of each group were subjected to image analysis on days 0, 3, 6, 9, 12 and 15
post amputation. The difference between the control group and the treatment exposed groups in the growth parameters such as caudal fin length increment and length gain percent were analysed. The statistical analysis of the experimental data revealed the variation in treatment means, which were then determined and statistically represented by repeated measures ANOVA, post hoc multiple comparison test (LSD) and independent sample t test. The result showed that the fishes in each treatment group reverted back to its pre amputated caudal fin length in shorter growth period compared to the control group (p<.01). The percentage mean caudal fin length increment was significantly different from control in Amalakyadi Rasayana and Amalaki (p<.05). The results of the study demonstrate the significant potential of two Rasayana yoga Amalakyadi Rasayana and Swadamstradi Rasayana, along with their constituent ingredients Amalaki, Tila, Swadamstra and Amruth, in promoting tissue regeneration in the amputated caudal fin of zebrafish. The knowledge could have implications for treating degenerative diseases in humans and could be applied to promote gene therapies or pharmaceutical interventions to promote regeneration
NAME OF SECOND PRESENTEE:DR. Dr.SIBINA T P
DISSERTATION TITLE: N-VITRO IMMUNOMODULATORY AND ANTIOXIDANT ACTIVITY OF DHATRI TILA RASAYANA
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
The term "immunomodulation" refers to the regulation and modulation of immunity, which may be accomplished by either enhancing or suppressing the immune response. A medication with immunomodulatory activity has particular importance given in the current state of pandemics, the rise in antibiotic resistance, and autoimmune diseases. immune cells depend on reactive oxygen species to maintain their functions, they require sufficient levels of antioxidant defenses in order to prevent the negative effects of excessive production of reactive oxygen species. so, it would be ideal if a drug has both immunomodulatory and antioxidant activities. Here lies the importance of Rasayana Tantra which consists of many formulations aiming at increasing the quality of life by imparting longevity, memory, disease-free life, youth, excellence of body, and sense organs. Dhatri Tila Rasayana mentioned in Madhava Chikitsa contains Dhatri, Tila, and Bhringaraja. For the study purpose, a hydroalcoholic extract of the formulation was prepared. To assess the antioxidant activity two experiments were done. Total phenolic content estimation was done by the spectrophotometric method using Folin-Ciocalteau reagent. The scavenging potential of the formulation was assessed by DPPH assay. To assess immunomodulatory activity, proinflammatory cytokines such as IL 1, IL 6, and TNF were measured on LPS-stimulated macrophages before and after treating them with the sample. Datri tila Rasayana significantly decreases proinflammatory cytokines in LPS-stimulated RAW 264.7 cells. It has also been shown to have significant antioxidant activity.
NAME OF THIRD PRESENTEE:DR. RAKESH P
DISSERTATION TITLE: DESIGN, DEVELOPMENT AND VALIDATION OF AN INSTRUMENT FOR THE PREPARATION OF KSHARA.
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Introduction
Kshara is a unique form of medicine mentioned in Samhithas. Kshara (alkaline substances),is an Ayurvedic medicament obtained from ash of one or more plants, animal and mineral products.Kshara is superior among Sastra and Anusastra because of its Chedana, Bhedana, Lekhana actions. It is also included in Upayantras. Kshara which is used properly by an intelligent person can cure conditions like poison, fire, sharp weapons, and dreadful diseases.
Methodology
During the production of various
medicinal formulations, different types of appliances are required. These
appliances are specially designed for specific processes. In classical
preparation of Kshara, Major difficulties faced during the preparation
of Kshara were, wastage of raw materials, low yield and required more
manpower and time. This study was an attempt to reduce these difficulties by
developing an instrument for the preparation of Kshara. At the
commencement of the study a survey was conducted among Ayurveda doctors who
prepared Kshara and Survey results were adopted for selecting the
methodology of the study. Also, Apamarga (Acharanthes aspera) Kshara was
selected for the study on the survey basis. The analytical parameters of the 
Apamarga Kshara prepared with the newly developed
instrument were compared with the classically prepared one.
Results
The results showed that yield from
the instrumental method was higher than the classical method and the pH was
equal. The Physcio chemical and Instrumental results of the Kshara
prepared with instrument was comparable with that of classical method.
Conclusion
This newly designed and developed
instrument was found effective in reduce
the man power and time taken for whole process and it also aided in increasing
the yield of Kshara.
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