INTIMATION FOR BODHIKA SEMINAR - 28/09/2022

DEPARTMENT OF RASA SHASTRA AND BHAISHAJYA KALPANA

GOVT. AYURVEDA COLLEGE, THIRUVANANTHAPURAM

NAME  OF  FIRST PRESENTEE: Dr Ranjini N R

THESIS TITLE:

ACUTE AND SUBACUTE TOXICITY OF 

RASAGARBHA POTTALI IN WISTAR RATS

NAME OF GUIDE: Dr  R. Rajam      

        

NAME OF CO-GUIDE: Dr Achuthan C R         

DATE:28/09/2022

TIME: 2:00-4:00 PM

VENUE: OFFLINE AT COLLEGE AUDITORIUM

ABSTRACT

Pottali Kalpana is a unique preparation mentioned in Ayurvedic texts. Rasagarbha pottali, the experimental drug is a formulation developed by Acharya Vasudev Mulasankara Dvivedi and Harisankar Sarma, first recorded in the book, Rasasastra by PHC Murthy. The present study was aimed at assessing the acute and sub-acute toxicity of orally administered Rasagarbha pottali. Rasagarbha Pottali was prepared in gandhaka dravapaka method. Further analyses like XRD, XRF and PSA done. Acute toxicity test was performed on Wistar rats at a single oral dose of 1000 mg/kg for 14 consecutive days. General behavioural adverse effects and mortality were determined. In the sub-acute study, Rasagarbha Pottali was administered orally at doses of 100, 150 and 200 mg/kg daily for 28 days to Wistar rats. Sections of brain, heart, lungs, livers, stomach, kidneys and reproductive organs were removed for histological studies. Acute toxicity study showed that the oral LD₅₀ value of RGP was above 1000 mg/kg. The sub-acute toxicity study did not produce any observable symptoms of toxicity and no significant variation in body weight, organ weights, haematological and biochemical parameters or mortality in all treated rats. Mercury was detected at levels below detection in the liver tissue of high-dose females via ICP-MS. It was concluded that drug RGP did not cause any systemic adverse effect in the acute and sub-acute oral toxicity trials in rats up on oral administration and is safe at all doses tested over a period of 28 days in rodents. Thus 100- 200 mg/Kg body weight of the RGP is safe to use in preclinical pharmacological efficacy analysis up to a month period.

NAME  OF  SECOND PRESENTEE: Dr Sini Elizabeth Sunny       

                               

THESIS TITLE: MARKET SAMPLE ANALYSIS OF 

KANCHANARA GUGGULU MANUFACTURED BY 

SELECTED GMP CERTIFIED AYURVEDIC

 COMPANIES IN KERALA

NAME OF GUIDE: Dr S.Tharalakshmi                                 

NAME OF CO-GUIDE: Dr C.V. Meena  

DATE:28/09/2022

TIME: 2:00-4:00 PM

VENUE: OFFLINE AT COLLEGE AUDITORIUM

ABSTRACT

Guggulu, an exudate obtained from the plant Commiphora mukul is a common medicinal ingredient widely used in ayurveda since time immemorial. Marketing of guggulu and its preparations is a great concern of different ayurvedic pharmaceutical industry due to its adulteration, irrational substitution and non-availability of genuine samples. Proper quality of the formulations is essential for maintaining its therapeutic efficacy. Also, standardisation of raw drugs and formulations with the help of modern analytical tools increase their scope, acceptance and scientific validity.

In the present study, an attempt was made for the market sample analysis of Kanchanara guggulu tablets manufactured by selected GMP certified ayurvedic companies in Kerala. Kanchanara guggulu is a classical formulation mentioned in Sharangadhara Samhita and Bhaishajya Ratnavali containing guggulu as its major ingredient. The other ingredients are Kanchanara twak, Sunti, Pippali, Marica, Haritaki, Vibhitaki, Amalaki, Varuna twak, Patra, Ela and Twak. No market sample analysis has been conducted on this potential medicine irrespective of the adulterations in guggulu and of other raw drugs like marica, nagara, ela etc.

The study was based on the standard analytical parameters proposed by API and PLIM.  6 samples of tablets were prepared as per references in Sh.Sam and B.R  following the same manufacturing procedures. 4 market samples were randomly selected from GMP certified pharmacies producing the tablets for analysis and comparison. Marked variations were observed in some of the analytical parameters of ash values and extractive values and also in the quantification of guggulsterone using HPTLC in prepared and market samples. Statistical analysis of the analytical parameters shown high statistical significance(p<0.01). Study proved the need of standardisation and quality control for maintaining proper efficacy of the formulations.

NAME  OF  THIRD PRESENTEE: Dr Saranya Bhadran

THESIS TITLE: 

EFFECT OF KOKILAKSHADI KASHAYA IN 

HYPERURICEMIA                                                                                                                                                                                                   

NAME OF GUIDE: Dr Sreeni. T.V

DATE:28/09/2022

TIME: 2:00-4:00 PM

VENUE: OFFLINE AT COLLEGE AUDITORIUM

ABSTRACT

Hyperuricemia is defined as plasma(serum) urate concentration > 6.8 mg/dL. The prevalence of hyperuricemia varies with age and gender. Over all prevalence rate of hyperuricemia is 25.8%. A higher proportion of males to have hyperuricemia than females. Effective management of hyperuricemia helps in preventing risk factors like gouty arthritis, nephrolithiasis and urate nephropathy. The yoga selected for the study is Kokilakshadi Kashaya mentioned for Vatharakta in Bhaishajya Ratnavali,is widely used by ayurvedic practitioners for treating hyperuricemia.

                                        Methodology includes literature review, preparation and analysis of drug, clinical study and statistical analysis. In this study clinical evaluation of hyperuricemic effect of Kokilakshadi Kashaya was done an interventional study pre and post test without control group in 20 subjects are selected according to the inclusion criteria from OPD of Govt Ayurveda college, Trivandrum. Kokilakshadi Kashaya was given to subject with a dose of 48 ml twice daily before food and 250mg of Pippali Choorna was given as Anupana. The duration of study was 45 days. The formulation Kokilakshadi Kashaya is effective in reducing serum uric acid level and symptoms of Gouty arthritis i.e. pain, swelling, redness. So Kokilakshadi Kashaya can be suggested as a good choice for Hyperuricemia since all the ingredients are easily available with less chance of adulteration and comparatively cheap.