INTIMATION FOR BODHIKA SEMINAR 27/09/2023
DEPARTMENT OF AGATHA THANTRA
GOVT. AYURVEDA COLLEGE, THIRUVANANTHAPURAM
NAME OF FIRST PRESENTEE: Dr. VIJAYALAKSHMI D
DISSERTATION TITLE:PHYSICOCHEMICAL EVALUATION OF KUPEELU SEED (STRYCHNOS NUXVOMICA Linn.) WITH DIFFERENT SODHANA METHODS
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Ayurveda is
one of the world’s oldest medical systems and remains as one of India’s
traditional health care systems. Most of the Ayurvedic formulations are herbal,
some of the formulation contain poisonous drugs also. It is essential to do the
sodhana of these toxic drugs before using it therapeutically. The concept of
sodhana not only means the removal of physical and chemical impurities but also
minimizing the side effects of the drugs and thereby making the drug capable
for pharmaceutical preparation. Kupeelu is described under upavisha varga whose
seeds have been used against a number of diseases after proper sodhana. It is a
spinal poison that acts particularly on the anterior horn cell of the spinal
cord. It is a CNS stimulant, producing excitation of all portions of the
nervous system. Convulsions are produced due to direct action on the reflex
Centre of the spinal cord and affects all muscles at a time. So, it is very
essential to remove the physicaland chemical impurities of kupeelu by proper
sodhana methods before using it therapeutically. Different sodhana methods for Kupeelu
have been mentioned in Ayurvedic classics such as Rasa tarangani,
Yogaratnakara, Kriyakoumadi etc. Various media have been mentioned in textbooks
such as godugdham, tanduliya swarasam, kataka kwatha, takra, gomutra, gogrutha
etc. In this study the media used for sodhana procedures were godugdham,
gogrutham, gomootram, takram, and kanjikam. Aim of the study is to establish
the physicochemical changes occurring in kupeelu after sodhana and also to
analyze the rawkupeelu sample with sodhita kupeelu in different media. After
sodhana procedures, chemical constituents were analyzed by Thin Layer
Chromatography (TLC). Then quantitative analysis was done by using High
Performance Thin Layer Chromatography (HPTLC). The quantities of Strychnine and
brucine before and after purification were analyzed with paired t test and the
comparison between each sample of purified kupeelu was made using one way
ANOVA. Significant decrease in the quantity of brucine was found in the kupeelu
purified in (Go mutram+ Go ksheeram), takram and Danyamlam (p<0.001).
Significant decrease in the amount of strychnine was observed in the Kupeelu
purified with go ksheeram, go mutram, Danyamlam, Takram, go ghrutham all
exhibit highly significant mean difference (p<0.001) incompared with Raw
materials.
NAME OF SECOND PRESENTEE: Dr. MANEESHA M
DISSERTATION TITLE: EFFECT OF PATOLADIGANA KASHAYA WITH VILWADI GULIKA AND SIGRUPUNARNAVADI LEPA IN THE MANAGEMENT OF PIT VIPER ENVENOMATION
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
India is one among the countries most dramatically affected by snake bite mortality in the world. Out of 3900 species of snake in the world, about 250 species occur in India, in which 52 are known to be venomous. Prominent venomous species have been traditionally labelled as the ‘big four’ that includes Cobra, Krait, Russell’s viper and Saw Scaled Viper. Among these, viper bite is responsible for majority of snake bites in India .Vipers are classified into pit vipers and pit less viper based on the presence or absence of Loreal pit. According to Ayurveda, morphological features, Signs and Symptoms of envenomation of pit vipers are correlated to Mandali sarpa. Kerala has sustained traditional background in visha chikitsa since decades. Wide variety of herbal and herbomineral preparations are explained in the Ayurvedic classics under Mandali visha chikitsa. Poly valent anti snake venom (PAV) is the main treatment indicated for big four, but in pit viper bites, ASV has no role in reducing its toxicity and there is no ideal protocol indicated for its management. Only symptomatic treatment is recommended for pit viper envenomation in modern system of medicine. So there is a need to address this condition with our traditional visha chikitsa. Here was a humble attempt to find out the Effect of Internal administration of Patoladigana kashaya with Vilwadi gulika and Sigrupunarnavadi lepa in the management of Pit viper envenomation. Study design was pre and post interventional test and sample size of 14 participants with history of identified pit viper envenomation belonging to the age group 18-70 satisfying the inclusion criteria, attending OPD of Pappinissery visha chikitsa kendram Kannur. Participants were examined and details recorded as per case proforma with prior informed consent. Freshly prepared Patoladigana kashaya was given 96 ml per day in 2 divided doses along with one Vilwadi gulika and Sigrupunarnavadi lepa externally at an interval of every 6 hour, for a period of 5 days. Symptomatic assessment of edema, pain, burning sensation and erythema were recorded before treatment, on 3rd day, after treatment (5th day) and after follow up(11th day).The outcome variables-Edema,pain,burning sensation and erythema were assessed for the changes in the mean score. Data analyzed statistically using non-parametric statistical test(Friedman’s test).The result obtained was highly significant in relieving the cardinal symptoms of pit viper envenomation.
NAME OF SECOND PRESENTEE: Dr. GOPIKA PARAMESWARAN
DISSERTATION TITLE:HEPATOPROTECTIVE EFFECT OF ROHITAKADI CHURNA IN PARACETAMOL INDUCED HEPATOTOXICITY IN ALBINO RATS
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
All over the world, millions of people are affected by serious liver disorders, which are very difficult to treat despite of many efforts. Liver disorders have been classified in the high priority areas of health care. Hepatotoxicity is one of the major reasons due to which drugs continue to be taken off from the market. In ayurveda liver has prime importance and liver disorders are coming under the purview of Kamala, Pandu, Sopha and Udara. Rohitakadi churna is mentioned in chakradatta in the context of liver disorders. It is indicated in Udara, Pliha, Prameha, Arsa, Krimi and Gulma. Acharya Charaka has explained that the descriptions given for Pliha are to be taken for Yakrit also, and the difference being in the site and side of the body. And no effort has been made scientifically to evaluate its hepatoprotective effect till date. So, the Rohitakadi churna was evaluated for hepatoprotective activity and its acute toxicity. Pharmacognostical and phytochemical evaluation were also done. Phytochemical evaluation revealed the presence of flavonoid, tannins, phenols, alkaloids and steroids. The hepatoprotective effect of drug in comparison with Silymarin was studied in Paracetamol induced hepatotoxicity in Albino rat. The experiment consists of 6 groups with 6 Albino rats in each group. Groups were positive control group (distilled water), standard group (Silymarin orally 100mg/kg), Negative control group (Paracetamol 2g/kg), therapeutic dose of Rohitakadi churna (0.108g/200g rat), double dose of Rohitakadi churna (0.216g/200g rat) and hydroalcoholic extract of Rohitakadi churna(500mg/kg). Standard drug and study drug were given for 7 days. On 7th day Paracetamol was given orally after 2 hours of the administration of drug except normal control. On 8th day animals were sacrificed and blood & liver samples were collected and subjected to biochemical and microscopical analysis respectively. The results were analysed by One-way ANOVA followed by Tukey HSD post hoc multiple comparison test. The paracetamol treatment caused significant elevation in serum level of SGOT, SGPT, ALP & serum bilirubin and reduction in total protein & albumin levels. However, the animals treated with standard drug and study drug did not show an elevation in the biochemical values as compared to paracetamol treated group(p<0.01). In liver histopathology, extent of damage was minimal and hepatic regeneration was noted in study drug treated group as compared to paracetamol treated group. Further a ‘limit test’ performed in accordance with OECD guideline 425(acute toxicity) indicated that dose upto 2000mg/kg is safe. Hence it was concluded that Rohitakadi churna and its hydro alcoholic extract have significant hepatoprotective effect and it can be developed into safe herbal hepatoprotective drug.
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