INTIMATION FOR BODHIKA SEMINAR - 7/2/2022
NAME OF GUIDE: DR A SHAHUL HAMEED
DATE: 07/02/2022
TIME: 2:00-4:00 PM
VENUE: MICROSOFT TEAMS
ABSTRACT
Cosmostigma racemosum(Roxb.)Wight is a green milky weed climber belonging to Asclepiadaceae family. The present study is conducted to evaluate the wound healing activity of the plant. The descriptions from Hortus Malabaricus and various ethnobotanical studies of the sacred groves of temples in Kannur district serve as the base of this study. Wound healing is a complex process, which involves haemostasis, inflammation, proliferation and remodelling with scar tissue formation. The mobilization and proliferation of fibroblasts and angiogenesis play a major role in wound healing. A potent wound healer aids the body tissues in these steps more effectively by reducing the healing time .
The pharmacognostical, physical and phytochemical analysis of Cosmostigma racemosum(Roxb.) Wight are done as these details are not yet scientifically validated. In the in vitro assay, the cell mobilization of fibroblasts will be monitored by Scratch assay of L929 cells. Angiogenetic potential of the drug will be assessed using CAM Assay model (Chick Chorioallantropic membrane model). The wound healing activity of the plant is studied in vivo in Wistar rats by Excision wound model. Excision wounds of approximately 450mm2 will be made on the dorsal side of pre anesthetised rats. Three groups containing six rats each are used for study. For group I topical serveas the negative control.For group II application of Povidone iodine is used which serve as the standard. For Group III topical application of leaf paste of Cosmostigma racemosum (Roxb.)Wight is used which serve as the test group. The application is once daily for 14 days. Assessment will be done on 0th , 2nd ,4th ,6th ,8th ,10th and 14th days. The rate of wound contraction, period of epithelisation and histopathological analysis are assessed by this model.
