INTIMATION FOR BODHIKA SEMINAR-31/01/2022
NAME OF GUIDE: DR PRASANTH S R
DATE: 31/01/2022
TIME:2:00-4:00 PM
VENUE: MICROSOFT TEAMS
The liver is a crucial organ for the maintenance of gastrointestinal homeostasis and body function in general. In addition to its role in the digestive process, it plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. Certain medicinal agents when taken in overdoses and sometimes even when introduced within therapeutic ranges may injure the liver and this chemical-driven liver damage is termed as hepatotoxicity. Liver injury due to prescription and non prescription medication is a growing medical, scientific and public health problem. Worldwide the estimated annual incidence rate of drug induced liver injury is 13.9-24.0 per 100000 inhabitants. Therefore a more objective and reproducible remedy is needed for the management of the crisis.
In Ayurveda clinical features resembling toxicity of different organ systems are dealt in many contexts. Gara visha is a vast concept mentioned in the Ayurvedic classics, which can be comparable with various sources of exposure of toxins in our daily life activities. Any form of incompatible drug combination or less potent toxins that may get into our body accidently or intentionally, will definitely harm the consumers. The concept of gara in Ayurveda holds well worse with the pathology of hepatotoxicity which need to be unfolded in the lights of our holistic approach. Several formulations are also mentioned in Ayurveda classics which are effective in managing toxicity including hepatotoxicity. Visaghna Mahakashaya has been mentioned in Charaka Samhita Sutra sthana 4th chapter Shadvirechanashathasritheeyam as a potent remedy for all toxins. It is selected for assessing the hepatoprotective action in paracetamol induced hepatic cells. Healthy HepG2 cells will be collected from NCCS Pune, cultured appropriately and hepatotoxicity will be induced with paracetamol. MTT, ROS and Annexin V / FITC Assays are carried out in controls and sample added specimens and observations will be statistically analysed.
This Invitro study of Visaghna Mahakashaya will help us to decipher the mode of action of this combination at the hepatocellular level, thereby exploring its scope for further clinical trials. Moreover all the drugs mentioned in this Mahakashaya is locally available and cost effective compared to the existing therapeutic measures.
Key words:
Hepatotoxicity, gara, invitro study, Visaghna Mahakashaya
