INTIMATION FOR BODHIKA SEMINAR 14/11/2023
DEPARTMENT OF RASASASTRA AND BHAISHAJYAKALPANA
NAME OF FIRST PRESENTEE:DR. SRUTHI S S
DISSERTATION TITLE: ANALYTICAL PROFILING OF GUDUCHYADI KASHAYA AND REPEATED EXTRACTS OF ITS RETENTATE.
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Most of the Ayurvedic formulations are
polyherbal in nature and phytoconstituents present in them are responsible for
their therapeutic activities. But nowadays herbal drug pharmaceutical
industries face many threats and challenges such as scarcity of raw drug
materials, substitution and adulteration, reduction in quality, and increase in
price of raw materials. Hence in this scenario, maximum utilisation of raw
drugs should be ensured while preparing the Ayurvedic medicines for the
preservation of biodiversity. The bioactive principles from herbal medicines
are extracted with water, oil, ghee, milk, etc. but recovery of active
principles into the extracted phase will not be complete. Therefore, the
retentate or spent material of Ayurvedic formulations will be a very rich
source of bioactive phytocompounds. This research study was conducted to determine the phytoconstituents
retained in the spent material of Guduchyadi Kashaya. Physicochemical and phytochemical comparisons
were done between Guduchyadi kashaya and subsequent kashayas
prepared from its retentate. For this purpose, physicochemical analysis,
chromatographic and spectroscopic techniques, mainly HPLC, LCMS/MS, NMR
spectroscopy, TPC, and TFC analysis, were carried out in six samples of
methanolic extract of lyophilized Guduchyadi Kashaya. As per the findings of this
research study, phytoconstituents are retained in the subsequent kashayas
prepared from the retentate of Guduchyadi Kashaya.The analysis using TPC,
TFC, and LC-MS/MS showed that the concentration of certain phenolic and
flavonoid compounds was increased in the subsequent kashayas. Statistically significant increase of berberine
content in subsequent kashayas was observed in HPLC analysis. So, it can be
concluded that retentate of Guduchyadi Kashaya contain phytoconstituents
with significant therapeutic potential.
NAME OF SECOND PRESENTEE:Dr.SANITHA U M
DISSERTATION TITLE: "INVITRO ANTIVIRAL ACTIVITY OF ARAGWADHADI KASHAYA IN H1N1"
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Influenza
A virus subtype H1N1, has posed a significant public health threat worldwide
due to its ability to cause seasonal epidemics and occasional pandemics. The
development of effective antiviral strategies is crucial to mitigate the impact
of H1N1 infections. Traditional medicine systems, such as Ayurveda, have long
been a source of potential remedies for various ailments. This study
investigates the in vitro antiviral activity of an Ayurvedic decoction against
the H1N1 influenza virus. Aragwadhadi
kashaya is a decoction having five ingredients Aragwadha
(Cassia fistula), Kanamoola (Piper longum), Mustha (Cyperus
rotundus), Tiktha (Andrographis paniculata) and Abhaya (
Terminalia chebula) as ingredients. It is indicated in fever associated
with Vata Kapha dosha and is mentioned in Sharngadhara samhitha. Fever
being the cardinal symptom of H1N1 a formulation indicated for fever is
selected.
The
ingredients of the decoction underwent preliminary physicochemical analysis for
identity, purity, and strength. The decoction was lyophilized and used for TLC,
HPTLC, FTIR, and in-vitro studies. Molecular docking involved screening 130
chemical constituents from the ingredients against Neuraminidase and
Haemagglutinin, with the top hits being Pectin and
(2R-2-[(3S)-3-Carboxy-5,6,7-trihydroxy-1-oxo-3,4-dihydroisochromen -4-yl
butanedioic acid) with scores of -6.2 and -7.36, respectively.
Initially,
MDCK cell lines were diligently prepared, ensuring their optimal growth and
health for subsequent experiments. Subsequently, virus isolation from throat
swab sample was carried out, with a focus on RNA isolation and PCR
amplification of the Matrix M gene to identify the influenza virus. To
determine the viral stock's concentration, a Haemagglutination Assay was
performed, providing valuable insights into its titration. To confirm the
infectivity of the virus, an Immunofluorescence assay was conducted.
Furthermore, a Plaque Assay was employed to quantify infectious virus particles
within the viral stock, aiding in accurate measurements. To assess the impact
of the virus, cytotoxicity was evaluated using both Haemolytic and MTS Assays.
Lastly, the anti-influenza activity was examined through a Neuraminidase
Inhibition Assay, which revealed no inhibition against Neuraminidase.
NAME OF THIRD PRESENTEE :Dr.ASHA KUMARI L S
DISSERTATION TITLE: "HEPATOPROTECTIVE EFFECT OF AMRUTHADI KASHAYA IN PARACETAMOL INDUCED LIVER DAMAGE IN WISTER ALBINO RATS."
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
In the current scenario, over two million people die from liver disease each year. There are only a few hepatoprotective drugs available in contemporary medicine. So there is a constant need for a safe hepatoprotective agent. Amruthadi kashaya mentioned in the Pandu Prakarana of Arogya Kalpadruma is indicated for Pandu, Kamala, and Raktha pitha.In this experimental study, the hepatoprotective effect of Amruthadi Kashaya was assessed using Paracetamol and compared with the standard drug Silymarin. The study was conducted using thirty six Wistar albino rats. The rats were divided into six groups of six each. Groups 1 and 2 were normal and control groups, respectively. Groups 3, 4, and 5 received Amruthadi kashaya in halftherapeutic, therapeutic, and double-therapeutic doses for seven days. Group 6 received Silymarin at a dose of 100 mg/kg orally for seven days. On the seventh day, 2 g/kg of Paracetamol was used to induce liver damage in groups 3 to 6. The hepatoprotective activity was evaluated by the biochemical parameters SGPT, SGOT, ALP, total protein, serum bilirubin, antioxidant enzyme SOD activity, and by analysis of the histopathology of the liver. The results were statistically analysed and compared between normal, standard, and treated groups. The therapeutic dose and double-therapeutic doses of kashaya controlled the expression of the enzymes within normal limits (SGOT: 19.23, 25.05, SGPT: 32.01, 24.74)and hence showed significant hepatoprotection.
No comments:
Post a Comment