INTIMATION FOR BODHIKA SEMINAR                                       11/10/2023

                             DEPARTMENT OF KAYACHIKITSA

GOVT. AYURVEDA COLLEGE, THIRUVANANTHAPURAM

NAME  OF  FIRST PRESENTEE: DR.ALCINA VARGHESE

DISSERTATION TITLE:EFFECT OF A SELECTED AYURVEDIC TREATMENT PROTOCOL IN DIABETIC PERIPHERAL NEUROPATHY

NAME OF GUIDE:   Dr. K. AMBIKA MD (AY)

DATE:11/10/2023

TIME: 2:00-4:00 PM

VENUE: COLLEGE AUDITORIUM

ABSTRACT

According to American Diabetes Association, Diabetic peripheral neuropathy (DPN) is ‘’the presence of symptoms or signs of peripheral nerve dysfunction in people with diabetes after exclusion of other causes”. DPN is the most common complication of diabetes mellitus. Globally diabetic neuropathy affects approximately 132 million people (1.9% of the population). DPN is by far the most common of all the diabetic neuropathies. Despite efforts to make an early diagnosis and to halt the progression of diabetic neuropathy, currently there is no effective treatment available at a global level.        In Ayurveda, a clear-cut clinical entity similar to DPN is not seen. When we look in to both, DPN may be considered as later stages of Prameha. Micro and macro vascular changes and neuronal ischemia are the main pathology in diabetic neuropathy which are similar to the rakta dushti and vatadushti .  Here abnormal neurological manifestations are associated with Prameha, a santarpanotha vyadhi, indicate the involvement of avarana pathology. So the study was undertaken in an effort to find an effective Ayurvedic treatment protocol to manage DPN and to provide a better quality of life. It included Deepana Pachana with Vachadi choornam and Nisakathakadi kashayam, Udwartanam with Kolakulathadi choornam, Vicharana snehapanam with Madhuyastyadi tailam in Nisakathakadi kashayam , Abhyanga with Bala tailam and  Usnambu snana , Virechana with Nimbamritaderanda tailam   followed by internal administration of  Vara, Vidanga, Krishna choornam with tailam, ghrita and madhu . Reduction of TCNS score and improvement in   Vibration Perception Threshold (VPT) assessed using Neuropathy Analyser was statistically analyzed and concluded. Statistical analysis showed that there was significant reduction of score in TCNS scale and improvement in VPT assessed through Neuropathy analyzer. Thus the study was concluded to be effective in reducing the signs and symptoms of DPN. NAME  OF  SECOND PRESENTEE: Dr. HARITHA.V.DAS DISSERTATION TITLE:ACTIVITY OF ASANADI KASHAYA IN GLUCOSE UPTAKE ON L6 CELL LINES AND HEPATIC GLUCONEOGENESIS ON HepG2 LIVER CELLS NAME OF GUIDE:   Dr MINI V.G M.D(AY) DATE:11/10/2023 TIME: 2:00-4:00 PM VENUE: COLLEGE AUDITORIUM

ABSTRACT

Diabetes mellitus is a unique disorder which involves right from DNA code in the genetics to daily diet of the individual. It has become such a world-wide problem that all the “pathies” of the medical science are putting effort to solve this problem. Insulin resistance is a core defect in type 2 diabetes. It contributes to hepatic glucose overproduction and is the major cause of decreased insulin-mediated glucose uptake by peripheral tissues.

Ayurveda, there is a description of Asanadi gana, mentioned in both Astanga hridaya and Astanga sangraha in the management of Switra, Kushta, Kaphaja vikaras, Krimi, Pandu, Prameha and Medodosha.

The main objective of the study was to assess the capacity of Asanadi kashaya to inhibit hepatic glucose production in HepG2 cells and Possible stimulative effect on glucose uptake in cultured L6 cell lines. The sample of Asanadi Kashaya is prepared as per the standard method mentioned in the sargdhara samhitha.

Cytotoxicity of cell line (L6 cell line) was determined by using MTT cell viable assay. The percentage of growth inhibition was calculated (percentage of viability). The glucose uptake activity was assessed in L6 cell lines. Glucose levels in the L6 cells were quantified using a glucose assay kit. Inhibition of hepatic gluconeogenesis by Asanadi Kashaya was assessed in HepG2 liver cells. the glucose content was estimated using sensitive glucose kit.

As per the findings, In Cytotoxicity Evaluation (MTT Assay) -result revealed Asanadi Kashaya displayed low level of toxicity to L6 cell lines. In glucose uptake assay, at a concentration of 100µg/mL the percentage of glucose uptake by the Asanadi kashayam was 67.10% as compared to other concentration of 25 µg/mL and 50 µg/mL. As the concentration increased, the percentage of glucose uptake was also increased. In Endogenous inhibition of glucose production in liver, the degree of inhibition reached a maximum of 71.80% at 6.25 µg/mL concentration under the test conditions and decreased at higher concentrations (i.e., 12.5 µg/mL and 25 µg/mL).

In this study Asanadi Kashaya has the anti-diabetic activity by inhibiting hepatic gluconeogenesis and stimulating glucose uptake in cell line

NAME  OF  THIRD PRESENTEE: Dr. K.NIMISHA NARENDRAN DISSERTATION TITLE:THE EFFECT OF MUNDITIKA CHOORNAM IN THE MANAGEMENT OF VATARAKTA WITH SPECIAL REFERENCE TO RHEUMATOID ARTHRITIS NAME OF GUIDE:   Dr.ASWATHY .S   M.D (AY) DATE:11/10/2023 TIME: 2:00-4:00 PM VENUE: COLLEGE AUDITORIUM

ABSTRACT

Vatarakta is a Vatavyadhi prabheda. Vatarakta possesses a special place in the literature, due to its high prevalence in the society, increased incidence as age advances, stepwise succession and so on. From the overall view of the etiology, it is obvious and unambiguous that the precise etiological factors of vata dosha as well as rakta dhatu are accountable for the causation of illness1. The umbrella of Vatarakta in parlence with conventional medicine includes many conditions related to extremities and to mention, a few are connective tissue disorders as well as peripheral vascular disorders. Vatarakta is one of the main articular disease which is characterised by severe pain, tenderness, inflammation and burning sensation in the affected joints. Rheumatoid arthritis is one of the most common chronic autoimmune anti inflammatory arthritis caused by symmetric polyarthritis and having destructive nature of varying extent. RA in its course, pathogenesis, signs and symptoms have many similar feature with Vatarakta.In the Kayachikitsa OPD of Govt Ayurveda College, majority of patients diagnosed as Vatarakta as per the lakshanas according to Ayurvedic classics shows symptoms similar to 2010 ACR criteria and favouring Rheumatoid Arthritis, so even though Vatarakta mimics the symptoms of Rheumatoid Arthritis, Systemic Lupus Erythematous, Sjogren’s ,Gouty Arthritis etc , Rheumatoid arthritis alone is taken for the study .

Study to be carried out may be helpful in the management of Rheumatoid Arthritis in Kayachikitsa OPD, Govt Ayurveda College Hospital Trivandrum. 22 patients fulfilling the inclusion and exclusion criteria diagnosed as Vatarakta according to the prevalidated tool regarding lakshanas of Vatarakta i.e ruk, sparshasahatva, daha, stabdhata, twak vaivarnya and as Rheumatoid arthritis by satisfying 2010 ACR diagnostic criteria were selected and subjected to detailed clinical examination according to clinical research proforma and lab investigation. The study drug Munditika choornam was given as 6gm bd with 1 teaspoon Goghrita, ½ teaspoon Madhu and 50 ml bd Guduchi kwatha ,to the study subjects for 30 days. Evaluation was done on 0th and 31st day. The results obtained were statistically analyzed and concluded