INTIMATION FOR BODHIKA SEMINAR 04/10/2023
DEPARTMENT OF DRAVYAGUNAVIGYAN
NAME OF FIRST PRESENTEE: Dr.HARITHA .N
DISSERTATION TITLE: GENUINENESS OF MARKET SAMPLES OF MUSTA( Cyperus rotundus Linn) IN KERALA
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Musta is a rhizomatous perennial herb that grows throughout the country, commonly found in waste grounds, gardens, and roadsides. The rhizome/tuber of Musta is a crucial ingredient in Ayurvedic formulations like Mustakadikwatha, Mustakarishta, Asokarishta etc, offering effective remedies for various ailments such as Jwara, Agnimandya, Ajirna, Swasakasa, Atisara, and more. The Ayurvedic Pharmacopoeia of India has identified the source plant of Musta as Cyperus rotundus Linn., belonging to the Cyperaceae family. Though Cyperus rotundus Linn. is widely present in Kerala, it is not extensively cultivated here. Additionally, there is a notable variation in the market price of Musta. This situation raises concerns about possible adulteration or substitution with lower-quality drugs or those from the same family, which can compromise the safety and efficacy of Ayurvedic formulations. To address these concerns, this study was conducted to determine the authenticity of Musta (Cyperus rotundus Linn.) used in Kerala. For this purpose, three sets of samples were collected from raw drug markets in each zone of Kerala North, Central, and South. Each set of sample included one from a rural market, one from an urban market, and one from a manufacturing unit. These samples were compared with genuine Musta and the standards outlined in the API (Ayurvedic Pharmacopoeia of India) through pharmacognostical and various physicochemical evaluations. Organoleptic evaluation revealed slight differences among the samples in terms of size, shape, external surface and internal colour. Results of phytochemical evaluation indicated marked differences between the market samples and the genuine sample in terms of foreign matter, volatile oil content, ash values, extractive values and fibre contents. Qualitative analysis also showed variations in the presence of steroids, phenols, glycosides, tannin and flavonoids among the samples. High-Performance Thin-Layer Chromatography (HPTLC) analysis revealed differences in peaks among the samples. All heavy metals screened were within permissible limits in all samples. Microbial examinations detected presence of mixed colonies of E. coli, Bacillus, and Klebsiella in all samples, and fungal growth of Aspergillus niger in two market samples. Overall, the study findings suggest that Musta available in herbal drug markets in Kerala are genuine but are of inferior quality.
NAME OF FIRST PRESENTEE: Dr. DIVYA P
DISSERTATION TITLE: WOUND HEALING ACTIVITY OF HARIDRADI YOGAM IN ALBINO RATS
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Wounds are the injuries and damages that happen to the skin since it’s the most accessible organ of human body. Wound healing can be considered as a physiological process in which a complex interaction of physical, chemical and cellular events take place. The most common preventable challenge to wound healing is possible infection, and topical antimicrobials have long been used empirically in an attempt to prevent wound infection. These agents support to prevent infection of the wounds but have no role in accelerating the natural healing process. The methods of wound healing in ayurveda are proven effective with anti-inflammatory and antiseptic properties with considerably lesser side effects in comparison to synthetic medicines that can even cause allergies. Wounds can be correlated to vrana and wound healing can be correlated to vrana ropana in ayurvedic terminology. Haridradi yogam is a taila kalpana mentioned in vrana chikitsa adhyaya of ‘Yogamritham’, a malayalam book written by D. Sreeman Namboothiri. It consists of Haridra (Curcuma longa), Yashtimadhu (Glycyrrhiza glabra), Sasasruthi (Emilia sonchifolia), Karpoora (Cinnamomum camphora), Madhuchishta (Bee wax) and Tila taila (Sesame oil) and is mentioned as vrana ropana.
Methodology : In-vivo excision wound experiment was done to assess the period of wound contraction, epithelialization period and histopathological parameters. Excision wounds of 450 mm2 area were made on the dorsal side of pre-anaesthetized rats. 3 groups containing 6 rats each were used for the study. Group I served as the untreated negative control. Group II served as the standard and Povidone iodine was topically applied. In group III topical application of the Haridradi yogam was done. The application was done once daily for 15 days. Wound measurements were taken on 0th day and then on every alternate days. In-vitro scratch assay was done using L929 cells to assess fibroblast proliferation and migration. CAM assay was performed using fresh fertilized eggs to assess the angiogenic potential of the yogam.
Results : Haridradi tailam showed significant wound contraction rate of 99.43+0.70 compared to standard drug povidone iodine (94.50+1.83) and control (92.16+0.93) on 14th day post-operation with p value <0.001. Period of epithelialization in days was least for the test group (8.5) compared to the standard (11) and control (12.67). In the histopathological analysis, the test group showed marked fibrosis, more collagen, granulation tissue and vascularization than the standard and control groups. The yogam also showed better percentage of wound contraction in scratch assay and better angiogenesis in CAM assay than the control groups.
NAME OF THIRD PRESENTEE: Dr MADHUBALA P GOPINATH
DISSERTATION TITLE: EFFICACY OF TRIPHALA PIPPALI YOGAM AS AN ADD- ON MEDICATION IN METABOLIC SYNDROME - A RANDOMIZED CONTROL TRIAL
TIME: 2:00-4:00 PM
VENUE: COLLEGE AUDITORIUM
ABSTRACT
Background: Metabolic syndrome (MetS) is an inter-related cluster of metabolic abnormalities involving glucose and lipid dysregulation, abdominal obesity, and elevated blood pressure and it is a major health problem worldwide. High calorie-low fiber diet, fast-foods, aging, sedentary lifestyle etc., trigger impaired glucose tolerance, insulin resistance, dyslipidemia, and inflammation. Several studies show that herbal supplements are moderately effective and safe as add-on medications in MetS.
Aim: To determine the efficacy of Triphalapippali yogam (TPY) in MetS
Materials & Methods: TPY is a polyherbal capsule (500mg) prepared by potentiating the fine powder of fruits of Haritaki (Terminalia chebula), Vibheetaki (Terminalia bellerica), Amalaki (Embelica officinalis) and Pippali (Piper longum) in its own decoction by wet grinding method.
Sixty-seven subjects diagnosed as MetS (as per Harmonization criteria 2009) with type 2 DM, attending OPD of Government Ayurveda college, Thiruvananthapuram, were randomized to receive 2 capsules of TPY (n = 34) or placebo (n = 33) two times daily for 12 weeks along with conventional Ayurveda add-on medication, Nishakathakaadi-tarpana-kashayam daily. The outcome measures were change in insulin resistance (HOMA-IR), fasting blood glucose (FBG), fasting insulin (FI), post-prandial glucose (PPBS) and glycosylated haemoglobin (HbA1c), lipid profile, body mass index (BMI), waist circumference (WC) and blood pressure and safety parameters. Analysis of covariance (ANCOVA) was used to model outcomes at 12 weeks, by treatment group corrected for baseline level of the outcome variable.
Results: Preliminary pharmacognostical, phytochemical and chromatographic profiling of individual ingredients of TPY matches with API parameters. The HPTLC profiling was developed with solvent system Toluene: Ethyl acetate: Formic acid (5:2:0.1). The preparation and standardisation of TPY was done as per CCRAS guidelines, which can be further utilised for large scale preparation of TPY.
HOMA-IR, FBS, PPBS, FI, HbA1c, TC, LDL-C, VLDL, Triglycerides, ALT, Weight, BMI, WC were reduced (p < 0.5) in subjects receiving TPY, compared to placebo after 12 weeks of treatment. The medicine was well tolerated and no adverse events were noted.
Discussion: TPY reduced MetS parameters as the ingredients having tridosha shamaka, rasayana and lekhana, pramehaghna and medohara properties. The active principles of TPY such as polyphenols, flavonoids, fibres, alkaloids have anti-oxidant, glucose and lipid lowering effects. The potentiation of the TPY by wet grinding reduced the particle size and encapsulation ensured better compliance in participants.
Conclusion: TPY showed significant improvements in several cardiovascular risk factors compared with placebo. TPY might be a promising alternative treatment for MetS and type 2 diabetes. TPY capsule is safe as it did not raise LFT and RFT values after 12 weeks.
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